姓名：王方昆

职称：副教授

邮箱：wangfangkun@sdau.edu.cn

办公室电话：18853857500

个人简介

    王方昆，博士，副教授，硕士生导师。中国微生物学会和中国免疫学会会员，中国畜牧兽医学会动物福利与健康养殖分会理事，山东免疫学会兽医免疫专业委员会委员。山东农业大学人才“筑峰”计划团队、山东省“黄河三角洲学者”团队和山东省牛产业技术体系疫病防控团队骨干成员。山东省科技特派员和山东省畜禽屠宰行业专家库和泰安市环境应急专家库医学专家。以第一作者或通讯作者在J. Bacteriol、Infect. Immun、Vet. Microbiol、Chemosphere、Environ. Pollut、和Poult. Sci 等英文期刊上发表论文30余篇。授权发明专利5项。参与制定沙门氏菌防控相关地方标准2项、中国兽医协会团体标准1项和山东省优质畜产品协会团体标准2项。

教育经历

2008 – 2011 , 农学博士 , 预防兽医, 中国农科院哈兽研所

2004 – 2007 , 农学硕士 , 基础病理, 山东农业大学动物医学院

2000 - 2004 , 农学学士 , 动物医学, 山东农业大学动物医学院

工作经历

2014 -至 今, 副教授 , 山东农业大学动物医学院

2015–2016 , 柏林自由大学 高级访问学者

2011- 2014 , 讲师 , 山东农业大学动物医学院

执教课程

兽医传染病学，人畜共患病学（本科生）

人畜共患细菌病学，动物传染病学与寄生虫病学和动物细胞培养技术（研究生）

研究领域

  1. 动物源人畜共患细菌致病分子机制与免疫机制；

2. 动物源人畜共患细菌的流行特征、耐药机制以及环境污染风险评估；

3. 新型益生菌活载体疫苗创制。 

承担的科研项目

    主持国家自然基金、国家重点研发计划（子课题）、山东省重点研发计划项目（子课题）、山东省农业重大应用技术创新项目（子课题）、山东省自然基金面上项目等国家、省部级项目十余项。

发表文章、专利

1. Sun, N., Y. Song, C. Liu, M. Liu, L. Yu and F. Wang^*(2021). Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Salmonella enterica serovar Typhmurium Isolated from Mouse Spleen. Pathogens 10(7).

2. Wang, F^*., W. Zhang and D. Niu^* (2021). Editorial: Foodborne Enterobacteriaceae of Animal Origin. Front Cell Infect Microbiol 11: 772359.

3. Wang, W., L. Yu, W. Hao, F. Zhang, M. Jiang^*, S. Zhao^*and F. Wang^*(2021). Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health. Front Public Health 9: 780700.

4. Liu, C., Y. Liu, C. Feng^*, P. Wang, L. Yu, D. Liu, S. Sun and F. Wang^*(2021). Distribution characteristics and potential risks of heavy metals and antimicrobial resistant Escherichia coli in dairy farm wastewater in Tai'an, China. Chemosphere 262: 127768.

5. Zhang, W., Z. Fu, H. Yin, Q. Han, W. Fan, F. Wang and Y. Shang (2021). Macrophage Polarization Modulated by Porcine Circovirus Type 2 Facilitates Bacterial Coinfection. Front Immunol 12: 688294.

6. Zhao, X., Z. Ju, G. Wang, J. Yang, F. Wang, H. Tang, X. Zhao and S. Sun (2021). Prevalence and Antimicrobial Resistance of Salmonella Isolated From Dead-in-Shell Chicken Embryos in Shandong, China. Front Vet Sci 8: 581946.

7. Liu, C., P. Wang, Y. Dai, Y. Liu, Y. Song, L. Yu, C. Feng, M. Liu, Z. Xie, Y. Shang, S. Sun and F. Wang^*(2021). Longitudinal monitoring of multidrug resistance in Escherichia coli on broiler chicken fattening farms in Shandong, China. Poult Sci 100(3): 100887.

8. Yu, L., Y. Liu, M. Liu, Z. Li, L. Li and F. Wang^*(2022). Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China. Poult Sci 101(5): 101763.

9. Li, Z., C. Peng, G. Zhang, Y. Shen, Y. Zhang, C. Liu, M. Liu and F. Wang^*(2022). Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China. Poult Sci 101(4): 101710.

10. Li, Z., L. Xin, C. Peng, C. Liu, P. Wang, L. Yu, M. Liu and F. Wang^*(2022). Prevalence and antimicrobial susceptibility profiles of ESBL-producing Klebsiella Pneumoniae from broiler chicken farms in Shandong Province, China. Poult Sci 101(9): 102002.

11. Liu, C., C. Feng, Y. Duan, P. Wang, C. Peng, Z. Li, L. Yu, M. Liu and F. Wang^*(2023). Ecological risk under the dual threat of heavy metals and antibiotic resistant Escherichia coli in swine-farming wastewater in Shandong Province, China. Environ Pollut 319: 120998.

12. Lv, P., X. Zhang, M. Song, G. Hao, F. Wang^*and S. Sun^*(2023). Oral administration of recombinant Bacillus subtilis expressing a multi-epitope protein induces strong immune responses against Salmonella Enteritidis. Vet Microbiol 276: 109632.

13. Su, Y., L. Xin, F. Zhang, C. Peng, Z. Li, C. Liu and F. Wang^*(2023). Drug resistance analysis of three types of avian-origin carbapenem-resistant Enterobacteriaceae in Shandong Province, China. Poult Sci 102(3): 102483.

14. Zhang, G., S. Li, Z. Shen^*and F. Wang^*(2023). Progress in research on the molecular biological detection techniques of avian encephalomyelitis. Res Vet Sci 159: 232-236.

15. Peng C, Zhang Y, Chen L, Li Z, Lv P, Wang P, Li N, F. Wang^*(2023). Bacillus subtilis expressing duck Tembusu virus E protein induces immune protection in ducklings. Microb Pathog. 185:106419.

16. Li Z, Peng C, Chen L, Wang P, F. Wang^*(2023). Construction and Immunogenicity Evaluation of Recombinant Bacillus subtilis Expressing HA1 Protein of H9N2 Avian Influenza Virus.  Curr Microbiol. 81(1):25.

17. Zhang Y, Wu Y, Peng C, Li Z, Wang G, Wang H, Yu L, F. Wang^*(2023). Both recombinant Bacillus subtilis Expressing PCV2d Cap protein and PCV2d-VLPs can stimulate strong protective immune responses in mice.  Heliyon. 9(12):e22941. 

专利

1. 王方昆等，马耳他型布氏杆菌的EF-Tu 蛋白单克隆抗体MAb及其制备方法与应用-国家发明专利，CN105218668A，2015.10.30

2. 王方昆等，一种沙门氏菌毒力基因及其应用-国家发明专利，CN202310174242.1， 2023.02.28

3. 王方昆等，一种布鲁氏菌特异性核酸检测方法，国家发明专利（申请号或专利号：202311092837.9），2023.08.29

4. 王方昆等，用于检测沙门氏菌属的特异性引物及其试剂盒-国家发明专利，CN112538543A，2020.12.16